We would like to do some proteomics. Can you help?

Hopefully! Take a look at the Collaboration page under the Research tab (coming soon). See if we are capable of helping you answer your particular biological question.

How long will it take to get my data back?

Because we work under a collaborative model and do not have core facility capacities, it can take a couple weeks to a couple months. There are many steps in the sample preparation and data analysis processes and we do our best to be as thorough as possible.

How much protein do I need? How many replicates?

The amount of protein needed depends on the type of experiment. Please see our Collaboration page (coming soon) or our Protocols Starting Point page for an idea of how to start.

We typically recommend triplicates, unless the fold changes are subtle, then quadruplicates will provide stronger statistical power. Duplicates can suffice if the fold changes are large.

What kind of quantitation do you use?

Depends on the type of experiment. We have extensive experience in various types of label-free quantification (MS1 level, DIA, etc.) as well as metabolic labeling like SILAC, and isobaric labeling (TMT). We will discuss our approach with you when planning the experiment.

Can you do metabolites/metabolomics?

Nope. Not until Maria sets it up….

How should we provide samples?

We typically ask that samples be provided at the final stage of the procedure. That is, frozen, washed cell pellets for proteome and PTM-profiling, or non-eluted beads for affinity purifications. In special cases we can perform the enrichments, but that can be a discussion.